However pre column derivatization has gained importance and can be achieved by a broader range of derivatization reagents.
Amino acid column hplc.
We are planning to develop method on analysis of amino acids in food matrix cereals pulses oil seeds etc by hplc with pre or post column derivatization methods since this is new to us so could.
The amino acid hplc column is a dedicated column developed by hawach for amino acid analysis.
The characteristics of the column will be affected by the chemical properties of this functional group.
It bonds with aminopropyl functional groups and through strict process control to ensure the formation of even dense amino functional groups.
In an amino column you have an nh2 group at the end of the carbon chain attached to the silica.
Optimized stationary phase for the separation of amino acids and other amine containing compounds.
Nh2 hplc columns description.
The use of internal and external standards is crucial.
Experimental conditions aaa on production 1200sl using eclipse plus c18 2 1 or 4 6 x 50mm 1 8u.
Whether you are looking to separate proteins peptides or amino acids the intrada series of columns has a solution for you.
Pre column derivatizing reagents most typically used for amino acid analysis are o phthalaldehyde phenyl isothiocyanate pitc fluorescamine and dansyl chloride.
Full chromatographic resolution of isobaric compounds like leu and ile.
Since most of the amino acids have no absorption in the ultraviolet.
Therefore pre column derivatization can be considered appropriate when intending to analyze a somewhat limited variety of samples with high sensitivity.
The nh2 group is a group where n has a lone e.
The nh2 hplc column uses the innovative filler bonding technology unique to hawach.
Post column derivatization was typical.
It uses ultra high purity fully porous spherical silica as a matrix to provide a good peak for amino acid analysis using a unique bonding process and a more thorough tailing process.
Amino acid analysis on eclipse plus c18 2 1 3 0 or 4 6 x 50mm 1 8µ column.
Hydrolysed samples amino acids are derivatized for sensitive detection separated by hplc.
It is based on ultra high purity and all hole spherical silica gel.
